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MB Sample ID: SA242964
| Local Sample ID: | am5_e_r1 |
| Subject ID: | SU002520 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002520 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| am5_e_r1 | SA242964 | FL030411 | evolved | age |
| am5_e_r1 | SA242964 | FL030411 | exponential | growth_phase |
Collection:
| Collection ID: | CO002513 |
| Collection Summary: | Bacterial cells were grown in DM25 medium and collected via vacuum filtration at 2hr and 24hr timepoints. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR002532 |
| Treatment Summary: | No treatment was applied to the samples. The samples consist of the ancestral strains of the LTEE, REL606 and REL607, and clones from each of the evolved lines, designated Ara-1:6 and Ara+1:6 |
Sample Preparation:
| Sampleprep ID: | SP002526 |
| Sampleprep Summary: | Samples were prepared using the 40:40:20 acetonitrile:methanol:water protocol. |
Combined analysis:
| Analysis ID | AN003957 | AN003958 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH002928 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B |
| Flow Rate: | 0.3mL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4 |
| Solvent B: | 20% water/80% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4 |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003692 |
| Analysis ID: | AN003957 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | EL-MAVEN |
| Ion Mode: | POSITIVE |
| MS ID: | MS003693 |
| Analysis ID: | AN003958 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | EL-MAVEN |
| Ion Mode: | NEGATIVE |
