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MB Sample ID: SA247819
| Local Sample ID: | PHP7 |
| Subject ID: | SU002565 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002565 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PHP7 | SA247819 | FL031167 | Control | treatment |
Collection:
| Collection ID: | CO002558 |
| Collection Summary: | The first pooped fecal sample in a day were collected during 3 days after the indicated time points and stored at -80 oC immediately for further research |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR002577 |
| Treatment Summary: | Out of those 60 students with anxiety and depression facing UNGEE, 30 were selected randomly as the PB group to receive the probiotic product; another 30 received placebo product were regarded as the P group. The 30 un-anxious students were assigned as the healthy control group (HP group). |
Sample Preparation:
| Sampleprep ID: | SP002571 |
| Sampleprep Summary: | Stool weighing 100 mg was added with 400 ml extract of methanol-acetonitrile-water (2:2:1, vol/vol/vol), vortexed and centrifuged at 14,000 g for 20min, and the supernatant was collected for further measurement. |
Combined analysis:
| Analysis ID | AN004042 | AN004043 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple quadrupole |
| MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Original peak intensity values | Original peak intensity values |
Chromatography:
| Chromatography ID: | CH002990 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | The gradient elution program was as follows: 0 --- 0.5 min, 95% B; 0.5 --- 7 min, B varied linearly from 95% to 65 %; 7 --- 8 min, B varied linearly from 65% to 40%; 8---9 min, B was maintained at 40%; 9---9.1 min, B varied linearly from 40% to 95%; 9.1--12 min, B maintained at 95%. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate; 25 mM ammonia |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003789 |
| Analysis ID: | AN004042 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | Ion Source Gas1（Gas1）：60，Ion Source Gas2（Gas2）：60，Curtain gas （CUR）：30，source temperature：600℃，IonSapary Voltage Floating （ISVF） The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003790 |
| Analysis ID: | AN004043 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Ion Source Gas1（Gas1）：60，Ion Source Gas2（Gas2）：60，Curtain gas （CUR）：30，source temperature：600℃，IonSapary Voltage Floating （ISVF） The raw data in Wiff format was converted to .mzXML format by ProteoWizard and then converted to .mzXML format using XCMS software. |
| Ion Mode: | NEGATIVE |
