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MB Sample ID: SA000049

Local Sample ID:mESC-T1R1
Subject ID:SU000004
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:Embryo
Species Group:Mammal

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Combined analysis:

Analysis ID AN000003
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290 Infinity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units Peak height

Chromatography:

Chromatography ID:CH000003
Chromatography Summary:Untargeted HILIC Method
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Pressure:300 Bar (Max)
Column Temperature:40
Flow Gradient:0-4 min - 0% B, 4-12 min - Gradient to 45% B, 12-20 min - 45% B
Flow Rate:0.25 mL/min
Injection Temperature:4 C
Sample Injection:5 uL
Solvent A:100% water; 0.2% acetic acid; 5 mM ammonium acetate
Solvent B:90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate
Analytical Time:20 min
Chromatography Type:HILIC
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