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MB Sample ID: SA001765
| Local Sample ID: | Control_3S |
| Subject ID: | SU000057 |
| Subject Type: | Invertebrate |
| Subject Species: | Caenorhabditis elegans |
| Taxonomy ID: | 6239 |
| Genotype Strain: | N2 |
| Age Or Age Range: | Young Adult |
| Gender: | Hermaphrodite |
| Species Group: | Invertebrate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000060 | AN000061 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Accela 1250 | Thermo Accela 1250 |
| Column | Thermo Scientific Gold aQ | Thermo Scientific Gold aQ |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000041 |
| Chromatography Summary: | Three microliters of each sample were injected onto a Thermo Scientific Gold aQ (150 × 2.1 mm, 1.9 μm) column using a column temperature of 40 °C and a flow rate of 600 μL/min with a gradient of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) from 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition. The inlet to the Orbitrap was held at a temperature of 320 °C, and the S-lens RF Level was set to 35%. The FR resolution was set to 70 000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm, externally calibrated. |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Thermo Scientific Gold aQ |
| Column Temperature: | 40 °C |
| Flow Gradient: | A (0.1% formic acid in water) and solvent B (0.1% formic acid in acetonitrile) from 100% solvent A for 1 min followed by a linear gradient to 20% B in 6 min, a linear gradient to 60% B in 2 min, a linear gradient to 95% B in 4 min, held for 2 min, and a 3.5 min return to the starting composition |
| Sample Injection: | 3 ?L |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 18.5 min |
| Chromatography Type: | Reversed phase |
