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MB Sample ID: SA005988
| Local Sample ID: | A02-30 |
| Subject ID: | SU000126 |
| Subject Type: | Animal |
| Subject Species: | Mirounga angustirostris |
| Taxonomy ID: | 9716 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000177 | AN000178 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | ||
| Column | ||
| MS Type | ESI | EI |
| MS instrument type | Triple quadrupole | Single quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap | Agilent 5973N |
| Ion Mode | POSITIVE | POSITIVE |
| Units | nM | nM |
Chromatography:
| Chromatography ID: | CH000121 |
| Methods Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 11 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
| Chromatography Type: | GC |
