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MB Sample ID: SA006108

Local Sample ID:C47
Subject ID:SU000134
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:13-wk-old
Gender:Male
Species Group:Mammal

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Combined analysis:

Analysis ID AN000195 AN000196
Analysis type MS MS
Chromatography type
Chromatography system
Column
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex API 5000 QQQ Thermo TSQ Quantum Ultra
Ion Mode NEGATIVE POSITIVE
Units uM uM

Chromatography:

Chromatography ID:CH000129
Chromatography Summary:Plasma free fatty acid concentrations were measured by liquid chromatography/mass spectrometry (LC/MS), as described previously (51). Briefly, 50 ?l of plasma was spiked with heptadecanoate internal standard (ISTD) and analyzed with Applied Biosystems (Foster City, CA) API5000 mass spectrometer coupled with a Cohesive (Franklin, MA) TX2 liquid chromatography system. Concentration of individual FFA was measured against a six-point standard curve prepared for each analyte. Both the ISTD and individual FFA standard curves were prepared in 2% fatty acid-free human albumin solution. All analytes were monitored as their [M ? H]? ions. LCFa-CoA esters were estimated using the LC-MS/MS method (9). After extraction in the presence of internal standard (20 ng of heptadecanoyl-CoA), samples were analyzed by UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters Acquity UHPLC, C8 UPLC BEH column 2.1 150 mm, 1.7 ?m (Waters, Milford, MA) and TSQ Quantum Ultra triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]. All standard curves were prepared using chemicals from Avanti Polar Lipids.
Methods Filename:PMID-24368672-Zabielski-Nair-AJPEM-2014.pdf
Chromatography Comments:Pubmed ID: 24368672
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