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MB Sample ID: SA006206
| Local Sample ID: | 041113_EcoliCells_Yjef_R4_0038 |
| Subject ID: | SU000138 |
| Subject Type: | Bacterial cells |
| Subject Species: | Salmonella enterica;Escherichia coli |
| Taxonomy ID: | 28901|562 |
| Genotype Strain: | DM14100|Salmonella enterica subsp. enterica serovar Typhimurium str. LT2|K 12 MG1655|K 12 MG1655 ridA3::tn10 ΔyoaB624::cat STM1549-26::kan| | |yjeF::kan |
| Species Group: | Microorganism |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000200 | AN000201 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000133 |
| Chromatography Summary: | Untargeted LC MS |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Pressure: | 300 bar (max) |
| Column Temperature: | 40 C |
| Flow Gradient: | 0 - 4 min: 0% B; 4-12 min: linear gradient to 45% B; 12-20 min: 45% B |
| Flow Rate: | 0.25 mL/min |
| Injection Temperature: | 4C |
| Sample Injection: | 5 uL |
| Solvent A: | 100% water; 0.2% acetic acid; 5mM ammonium acetate |
| Solvent B: | 90% acetonitrile/10% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Oven Temperature: | H20 + 5mM NH4OAc + 0.2% Acetic Acid |
| Time Program: | 20 min |
| Weak Wash Solvent Name: | 50:50 Methanol:Water |
| Weak Wash Volume: | 15 seconds |
| Target Sample Temperature: | Autosampler temperature 4C |
| Randomization Order: | Excel generated |
| Chromatography Type: | HILIC |
