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MB Sample ID: SA011878
| Local Sample ID: | WT_M1b |
| Subject ID: | SU000278 |
| Subject Type: | Bacterial cells |
| Subject Species: | Sinorhizobium meliloti |
| Taxonomy ID: | 382 |
| Genotype Strain: | Rm2011 (SU47 str-3)|SmA818|RmP3009|RmP2917 |
| Human Ethnicity: | wild type|Rm2011 lack pSymA|Rm2011 lack pSymB|Rm2011 lack pSymA and pSymB | a tRNAarg and the smb20996-engA operon from pSymB were integrated to chromosome in strains RmP3009 and RmP2917 |
| Species Group: | Bacteria |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000409 | AN000410 |
|---|---|---|
| Chromatography ID | CH000290 | CH000290 |
| MS ID | MS000351 | MS000352 |
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1200 RR Series II | Agilent 1200 RR Series II |
| Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker MicrOTOF II | Bruker MicrOTOF II |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | normalized peak area | normalized peak area |
Chromatography:
| Chromatography ID: | CH000290 |
| Methods Filename: | Opt_silicaHILIC.m |
| Instrument Name: | Agilent 1200 RR Series II |
| Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A |
| Flow Rate: | 200 ?L/min |
| Internal Standard: | L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine |
| Sample Injection: | 2 ?L |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid |
| Analytical Time: | 24 min |
| Chromatography Type: | HILIC |
