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MB Sample ID: SA012534
| Local Sample ID: | 248 |
| Subject ID: | SU000304 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-88 |
| Gender: | Femal(116), Male(118) |
| Human Smoking Status: | 67 nonsmokers and 167 smokers |
| Human Alcohol Drug Use: | 14 no alcohol and 220 alcohol |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000452 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | SeQuant ZIC-cHILIC |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | cps |
Chromatography:
| Chromatography ID: | CH000323 |
| Chromatography Summary: | The LC system was composed of two Agilent 1260 binary autosampler, and Agilent 1290 column valve (Agilent 10 ?L for analysis using negative ionization mode and 2 positive ionization mode. Both chromatographic hydrophilic interaction ZIC-cHILIC KGaA, to perform the separation, while the other column for the next injection. The flow temperature was kept the phase was composed of Solvents A (5 mM acetonitrile +0.2% acetic acetonitrile/ confirmed by spiking the with metabolites). However, some metabolites that could had similar m/z values (<1 Da) were acid and 3- samples not undergo any significant shift (each peak was of analysis), which proved the |
| Instrument Name: | Agilent 1260 |
| Column Name: | SeQuant ZIC-cHILIC |
| Column Temperature: | 40oC |
| Flow Gradient: | 0-2 min: 90%B; 2-5 min: to 50%B; 5-9 min: 50%B |
| Flow Rate: | 0.3 mL/min |
| Injection Temperature: | 4oC |
| Sample Injection: | 2 uL for positive and 10 uL for negative |
| Solvent A: | 90% water/10% acetonitrile; 0.2% acetic acid; 5 mM ammonium acetate |
| Solvent B: | 90% acetonitrile/ 10% water; 0.2% acetic acid; 5 mM ammonium acetate |
| Analytical Time: | 18 min |
| Capillary Voltage: | 3.8kV |
| Chromatography Type: | HILIC |
