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MB Sample ID: SA013078
| Local Sample ID: | 13 |
| Subject ID: | SU000306 |
| Subject Type: | Animal cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57/Bl6 |
| Subject Comments: | Primary mouse myocytes were used for this study. The cells were obtained from gastrocnemius muscle of male 8 weeks-old C57BL/6J mice. |
| Species Group: | Mammal |
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Combined analysis:
| Analysis ID | AN000454 | AN000455 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000: | Thermo Scientific-Dionex Ultimate 3000: |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH000324 |
| Chromatography Comments: | UHPLC, Thermo Scientific-Dionex Ultimate 3000: While setting up sequence, ensure that method to be utilized is PFP-metabolomics-neg350-NoBuffer-0-SID and PFP-metabolomics-neg-350-dd-MS2 for negative sequences. For positive sequences utilize PFP-metabolomics-pos-350-0-SID and PFP-metabolomics-pos-350-dd-MS2. Check the lines for air bubbles and purge line if present. Set injection volume to 2uL for positive samples and 4uL for negative samples. |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000: |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
