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MB Sample ID: SA022572
| Local Sample ID: | sample42 |
| Subject ID: | SU000464 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Combined analysis:
| Analysis ID | AN000694 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 |
| Column | None |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | micromolar |
Chromatography:
| Chromatography ID: | CH000502 |
| Chromatography Summary: | The derivatized extracts were analyzed with an Agilent 6890 gas chromatograph coupled with an Agilent 5973 mass selective detector. The 1-μL aliquots of the extracts were injected into a DB5-MS capillary column (30 m × 250 μm i.d., 0.25-μm film thickness; J&W Scientific, Folson, CA) using PTV injection (Gerstel CIS4 injector) in the splitless mode. The temperature of the PTV was 70 °C during injection, and 0.6 min after injection, the temperature was raised to 300 °C at a rate of 2 °C/s and held at 300 °C for 20 min. The initial GC oven temperature was 70 °C, 5 min after injection the GC oven temperature was increased with 5 °C/min to 320 °C and held for 5 min at 320 °C. Helium was used as a carrier gas and pressure programmed such that the helium flow was kept constant at a flow rate of 1.7 mL/min. Detection was achieved using MS detection in electron impact mode and full scan monitoring mode (m/z 15−800). The temperature of the ion source was set at 250 °C and that of the quadrupole at 200 °C. |
| Instrument Name: | Agilent 6890 |
| Column Name: | None |
| Chromatography Type: | GC |
