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MB Sample ID: SA025746

Local Sample ID:Sa_pre_35
Subject ID:SU000517
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Combined analysis:

Analysis ID AN000762
Analysis type MS
Chromatography type GC
Chromatography system Shimadzu GCMS-QP2010 ultra
Column a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA)
MS Type EI
MS instrument type GC-TOF
MS instrument name Shimadzu QP2010 Ultra
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000548
Chromatography Summary:The device used was GCMS-QP2010 ultra (Shimadzu Inc., Kyoto, Japan) equipped with a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) and AOC-20i (Shimadzu) as an autosampler. The injection temperature was 230 °C. The helium gas flow rate through the column was 1.12 ml/min. The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min. The transfer line and the ion source temperatures were 250 °C and 200 °C, respectively. Ions were generated by a 70 kV electron impact (EI), and 20 scans/sec were recorded over the mass range 85-500. A standard alkane mixture (C8-C40) was injected at the beginning of the analysis for subsequent identification of metabolites.
Instrument Name:Shimadzu GCMS-QP2010 ultra
Column Name:a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA)
Column Temperature:The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min.
Flow Rate:The helium gas flow rate through the column was 1.12 ml/min.
Sample Injection:1 µL
Chromatography Type:GC
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