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MB Sample ID: SA026976
| Local Sample ID: | 2045897 |
| Subject ID: | SU000537 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
| Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
| Gender: | Male| Male| Male| Male| Male| Male| Male |
| Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000788 | AN000789 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo QExactive | Thermo QExactive |
| Column | Higgins C18 (100 x 2.1mm,5um) | Higgins C18 (100 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak intensity | peak intensity |
Chromatography:
| Chromatography ID: | CH000565 |
| Chromatography Summary: | untargeted chromatography (C18 column) |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Chromatography Comments: | Thermo QExactive coupled to CTC PAL HTC Accela1-TMO autosampler |
| Instrument Name: | Thermo QExactive |
| Column Name: | Higgins C18 (100 x 2.1mm,5um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 0-2mins = 350 μL/min, 2-7mins = gradient to 400 μL/min, 7-10mins = 400 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Caffeine, Diethyl-toluamide |
| Internal Standard Mt: | 1.5 min; 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Weak Wash Volume: | 20μL |
| Chromatography Type: | Reversed phase |
