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MB Sample ID: SA032628
| Local Sample ID: | 1968432 |
| Subject ID: | SU000615 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Genotype Strain: | N/A|6.53kg|6.87kg|6.09kg|7.23kg|6.48kg |
| Gender: | Male| Male| Male| Male| Male| Male |
| Animal Animal Supplier: | none|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center| |Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/11|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle |
| Animal Inclusion Criteria: | Weight |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000907 | AN000908 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Ion exchange |
| Chromatography system | Thermo Accela CTC | Thermo Accela CTC |
| Column | Higgins C18 (100 x 2.1mm,5um) | Hamilton Anion Exchange (100 x 2.1mm,7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | unspecified | unspecified |
Chromatography:
| Chromatography ID: | CH000644 |
| Chromatography Summary: | untargeted chromatography (C18 column) |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Higgins C18 (100 x 2.1mm,5um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 350 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Caffeine, Diethyl-toluamide |
| Internal Standard Mt: | 1.5 min; 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000645 |
| Chromatography Summary: | anion exchange chromatography |
| Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
| Instrument Name: | Thermo Accela CTC |
| Column Name: | Hamilton Anion Exchange (100 x 2.1mm,7um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A%: 0-2mins = 5%, 2-7mins = gradient to 50%, 7-10mins = 50%; B%: 0-10mins = 50%; C%: 0-2mins = 45%, 2-7mins = gradient to 0%, 7-10mins = 0% |
| Flow Rate: | 350 μL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Cystine; cysteine-glutathione disulfide; carboxymethyl-cysteine (very low); glutathione disulfide (+1 and +2); carboxymethyl-glutathione |
| Internal Standard Mt: | 1-1.5 min; 2-4 min; 3.5-5 min; 7-8 min; 7-8 min |
| Sample Injection: | 10μL |
| Sampling Cone: | ESI: HESI probe with S-lens combination |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Running Voltage: | 4.6 kV |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Ion exchange |
