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MB Sample ID: SA033021
| Local Sample ID: | 2190631 |
| Subject ID: | SU000622 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Weight Or Weight Range: | NA|6.94kg|9.04kg|8.03kg|8.83kg|8.68kg |
| Gender: | Male|Male|Male|Male|Male|Male |
| Animal Animal Supplier: | Yerkes Field Station|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center||Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/12|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle |
| Animal Inclusion Criteria: | Weight|Weight|Weight|Weight|Weight|Weight |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000917 | AN000918 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Ion Intensity | Ion Intensity |
Chromatography:
| Chromatography ID: | CH000654 |
| Chromatography Summary: | C18 120k resolution method with C18 positive and negative wash |
| Methods Filename: | Metab_HFQE_Setup.docx |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Column Pressure: | 200 Bar (max) |
| Column Temperature: | 30 |
| Flow Gradient: | A: 0-2mins = 80%, 2-7mins = gradient to 0%, 7-10mins = 0%. B: 0-2mins = 15%, 2-7mins = gradient to 95%, 7-10mins = 95%. C: 0-10mins = 5% |
| Flow Rate: | 350μL/min |
| Internal Standard: | Cystine; cysteine-glutathione disulfide; carboxymethyl-cysteine (very low); glutathione disulfide (+1 and +2); carboxymethyl-glutathione |
| Internal Standard Mt: | 1.5 min, 6-6.6 min |
| Sample Injection: | 10μL |
| Sampling Cone: | HESI probe with S-lens combination for ESI |
| Solvent A: | 100% water |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 10 minutes |
| Capillary Voltage: | 4.6 kV |
| Oven Temperature: | 45C |
| Weak Wash Solvent Name: | H20 with 10% methanol |
| Sample Syringe Size: | 20μL |
| Chromatography Type: | Reversed phase |
