Return to study ST000601 main page
MB Sample ID: SA033183
| Local Sample ID: | 10473X |
| Subject ID: | SU000624 |
| Subject Type: | Human Plasma |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 57-70 |
| Gender: | Males and Females |
| Human Race: | Non-Hispanic White |
| Human Smoking Status: | Current and Former Smokers |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000920 | AN000921 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | HILIC |
| Chromatography system | Agilent 6210 | Agilent 6520 |
| Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | TOF | QTOF |
| MS instrument name | Agilent | Agilent |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Count | Counts |
Chromatography:
| Chromatography ID: | CH000656 |
| Chromatography Summary: | Lipid Fraction (C18) |
| Instrument Name: | Agilent 6210 |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Column Temperature: | 60°C |
| Flow Gradient: | 0-0.5 minutes 30-70% B, 0.5-7.42 minutes 70-100% B, 7.42-9.9 minutes 100% B, 9.9-10.0 minutes 100-30% B, followed by column re-equilibration |
| Flow Rate: | 0.7 mL/min |
| Injection Temperature: | 4°C |
| Sample Injection: | 4µL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 60% isopropanol/36% acetonitrile/4% water; 0.1% formic acid |
| Capillary Voltage: | 4000V |
| Randomization Order: | Excel rand() function |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000657 |
| Chromatography Summary: | Aqueous Fraction (HILIC) |
| Instrument Name: | Agilent 6520 |
| Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Column Temperature: | 20°C |
| Flow Gradient: | 0.2 minutes 100% B, 2-2.1 minutes 100-90% B, 2.1-8.6 minutes 90-50% B, 8.6-8.7 minutes 50-0% B, 8.7-14.7 minutes 0% B, 14.7-14.8 minutes 0-100% B, followed by column re-equilibration |
| Flow Rate: | 0.6 mL/min |
| Injection Temperature: | 4°C |
| Sample Injection: | 1µL |
| Solvent A: | 50% acetonitrile/50% water; ammonium acetate, pH 5.8 |
| Solvent B: | 90% acetonitrile/10% water; pH 5.8 ammonium acetate |
| Capillary Voltage: | 4000V |
| Randomization Order: | Excel rand() function |
| Chromatography Type: | HILIC |
