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MB Sample ID: SA052454
| Local Sample ID: | 4630 PB1-PHZ |
| Subject ID: | SU000927 |
| Subject Type: | Animals |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57/B6 |
| Age Or Age Range: | 8-16 weeks |
| Species Group: | Mammal |
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Combined analysis:
| Analysis ID | AN001452 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap |
| Ion Mode | POSITIVE |
| Units | peak area ratio |
Chromatography:
| Chromatography ID: | CH001021 |
| Chromatography Summary: | For DMAPA derivatized Fatty acid analysis. Shimadzu 20AD two pump system was used. Solvent A: 0.1% formic acid in water. Solvent B: 0.1% in Acetonitrile. The initial LC condition was 30% B. The solvent gradient was programmed from 30% B to 99% B in 4 min and was hold for 1 min at the flow rate of 1 mL/min. Then, it was immediately brought back to 30 % B and was hold for 2 min. An Agilent Eclipse XDB-C18 column (3.0 x100 mm x 3.5 µm) was used for this analysis. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um) |
| Column Temperature: | 60 C |
| Flow Gradient: | 30% B to 99% B in 4 min |
| Flow Rate: | 1 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 7 min |
| Chromatography Type: | Reversed phase |
