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MB Sample ID: SA055263
| Local Sample ID: | 14 |
| Subject ID: | SU000962 |
| Subject Type: | Cell culture |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Cell Strain Details: | C57BL/6 |
| Cell Primary Immortalized: | AT1 |
| Cell Counts: | ~60% confluency 6 well plate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001517 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | GCs connected in series are both from J&W/Agilent (part 122-5512) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | Peak values (Log transformed) |
Chromatography:
| Chromatography ID: | CH001070 |
| Chromatography Summary: | Metabolites were made volatile with trimethylsilyl (TMS) groups using N-methyl- N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50 C. GC/MS methods generally follow those of Roessner et al. (Roessner et al. 2000), Fiehn et al. (2008), and Kind et al. (2009), and used a 6890N GC connected to a 5,975 inert single-quadrupole MS (Agilent Technologies, Santa Clara, CA). The two wall-coated, open-tubular (WCOT) GC columns connected in series are both from J&W/Agilent (part 122-5512), DB5- MS, 15 m in length, 0.25 mm in diameter, with an 0.25-lm luminal film. Positive ions generated with conventional electron-ionization (EI) at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
| Instrument Name: | Agilent 6890N |
| Column Name: | GCs connected in series are both from J&W/Agilent (part 122-5512) |
| Chromatography Type: | GC |
