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MB Sample ID: SA058608
| Local Sample ID: | S7_1 |
| Subject ID: | SU001011 |
| Subject Type: | Other |
| Subject Species: | Papio hamadryas |
| Taxonomy ID: | 9557 |
| Age Or Age Range: | 18 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001592 | AN001593 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | Leco Pegasus 4D GC | Thermo Trace 1310 |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) | Thermo Scientific TraceGOLD TG-5SILMS (30m x 0.25 mm,0.25um) |
| MS Type | EI | EI |
| MS instrument type | GC x GC-TOF | Orbitrap |
| MS instrument name | Leco Pegasus 4D GCxGC TOF | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Arbitrary units | Arbitrary units |
Chromatography:
| Chromatography ID: | CH001117 |
| Chromatography Summary: | 1µL of derivatized sample into a split/splitless (SSL) injector at 250 °C using a splitless injection on a Thermo Scientific™ TRACE™ 1310 GC. Helium carrier gas at a flow rate of 1 mL/min was used for separation on a Thermo Scientific™ TraceGOLD™ TG-5SILMS 30 m length × 0.25 mm i.d. × 0.25 µm film thickness column. The initial oven temperature was held at 70 °C for 4 min, followed by an initial gradient of 20 °C/min ramp rate. The final temperature was 320 °C and held for 8 min. |
| Chromatography Comments: | 2D GC |
| Instrument Name: | Leco Pegasus 4D GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 250 |
| Internal Standard: | Adonitol, d4-succinic acid |
| Sample Injection: | 1 uL |
| Running Buffer: | Helium |
| Transferline Temperature: | 250 |
| Randomization Order: | Yes |
| Chromatography Type: | GC |
| Chromatography ID: | CH001118 |
| Chromatography Summary: | Samples were injected in splitless mode using an autosampler (VCTS, Gerstel™, Linthicum, MD, USA) consisting of an Agilent© 7890 B gas chromatograph (Agilent Technologies, Palo Alto, CA, USA) in line with a Pegasus ® 4D ToF-MS instrument (Leco Corp., San Jose, CA, USA) equipped with an electron impact (EI) ionization source. Injection temperature was set at 250 °C (front inlet) and the helium (carrier gas) flow rate was set to 1 mL min-1. Separation on the GC was achieved using two columns, a primary Rxi®-5Sil MS capillary column (Cat. No. 13623-6850, Restek, Bellefonte, PA, USA) (30 m × 0.25 mm × 0.25 μm) in line with a secondary Rxi®-17Sil capillary column (Cat. No. 40201-6850, Restek, Bellefonte, PA, USA) (2 m × 0.15 mm × 0.15 μm). The temperature program for the primary column started isothermal at 70 °C for 1 min followed by a 6 °C min-1 ramp to 310 °C and a final 11 min hold at 310 °C. The secondary oven temperature was programmed with an offset of 5°C whereas the modulator temperature offset was 15° C relative to the first oven temperature. The modulation temperature (second-dimension separation time) was 4 s divided into a hot and cold pulse times of 0.60 s and 1.4 s, respectively between the two stages. |
| Instrument Name: | Thermo Trace 1310 |
| Column Name: | Thermo Scientific TraceGOLD TG-5SILMS (30m x 0.25 mm,0.25um) |
| Injection Temperature: | 250 |
| Internal Standard: | Adonitol, d4-succinic acid |
| Sample Injection: | 1 uL |
| Running Buffer: | Helium |
| Transferline Temperature: | 250 |
| Randomization Order: | Yes |
| Chromatography Type: | GC |
