Return to study ST001062 main page
MB Sample ID: SA071866
| Local Sample ID: | B1_41 |
| Subject ID: | SU001106 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Age Or Age Range: | 3-4 weeks |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001736 | AN001737 | AN001738 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | AU | AU | AU |
Chromatography:
| Chromatography ID: | CH001228 |
| Chromatography Summary: | HILIC chromatography 17 minute run. Positive and Negative mode. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | The gradient started at 100% B for 2 minutes, then brought to 70% B by 7.7 minutes, 40% B by 9.5 minutes, back to 100% B by 12.75 minutes and held at 100% B until 17 minutes. |
| Flow Rate: | 0.4 mL/min |
| Internal Standard: | 5ug Val-Tyr-Val+ |
| Sample Injection: | 5uL |
| Solvent A: | 100% water; 0.125% formic acid; 10 mM ammonium formate |
| Solvent B: | 95% acetonitrile/5% water; 0.125% formic acid; 10 mM ammonium formate |
| Washing Buffer: | Water:ACN 50:50 |
| Target Sample Temperature: | 4 |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001229 |
| Chromatography Summary: | CSH C18 lipidomics run. Positive mode. As in Cajka et al. 2017 "Validating Quantitative untargeted lipidomics across nine liquid chromatography-High-Resolution Mass spectrometry platforms" |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | : 0 min 15% (B); 0−2 min 30% (B); 2−2.5 min 48% (B); 2.5−11 min 82% (B); 11−11.5 min 99% (B); 11.5−12 min 99% (B); 12−12.1 min 15% (B); and 12.1−15 min 15% (B) |
| Flow Rate: | 0.6 mL/min |
| Internal Standard: | [LPE(17:1), LPC(17:0), PC(12:0/13:0), PE(17:0/17:0), PG(17:0/17:0), d7-cholesterol, SM(d18:1/17:0), Cer(d18:1/17:0), sphingosine (d17:1), DG(12:0/12:0/0:0), DG(18:1/2:0/0:0), and d5-TG- (17:0/17:1/17:0)] |
| Sample Injection: | 3uL |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Washing Buffer: | IPA |
| Target Sample Temperature: | 4 |
| Chromatography Type: | Reversed phase |
