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MB Sample ID: SA072007
| Local Sample ID: | Adipose_59_young |
| Subject ID: | SU001110 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J mice |
| Age Or Age Range: | 8 week (“young”) and 112 week (“old”). |
| Weight Or Weight Range: | young: 20.2g (mean), aged: 34.3g (mean) |
| Gender: | Male |
| Animal Animal Supplier: | CLEA (Tokyo, Japan) |
| Animal Light Cycle: | a 12-hour light/12-hour dark schedule |
| Animal Feed: | CE-2, CLEA |
| Animal Water: | tap water |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001744 | AN001745 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Orbitrap XL | Orbitrap XL |
| Column | Waters XBridge C18 (50 x 2.1mm,3um) | Waters XBridge C18 (50 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ XL | Thermo LTQ XL |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001232 |
| Chromatography Summary: | The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1]) |
| Instrument Name: | Orbitrap XL |
| Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
| Solvent A: | 100% water; 0.01% acetic acid; 1 mM ammonia |
| Solvent B: | 75% ethanol/25% isopropanol; 0.001% acetic acid; 0.2 mM ammonia |
| Chromatography Type: | Reversed phase |
