Return to study ST001115 main page

MB Sample ID: SA076196

Local Sample ID:M_P9_5
Subject ID:SU001161
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Animal Animal Supplier:The Jackson Laboratory

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN001811
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela 600
Column Thermo Acclaim C30 (150 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units main area

Chromatography:

Chromatography ID:CH001278
Chromatography Summary:Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min.
Instrument Name:Thermo Accela 600
Column Name:Thermo Acclaim C30 (150 x 2.1mm,3um)
Column Temperature:30
Flow Gradient:35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min
Flow Rate:260 µL/min
Solvent A:60% methanol/40% water; 0.2% formic acid; 10 mM ammonium acetate
Solvent B:60% methanol/40% chloroform; 0.2% formic acid; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  logo