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MB Sample ID: SA080939

Local Sample ID:0.1_NC_96h_5
Subject ID:SU001235
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable

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Combined analysis:

Analysis ID AN001935
Analysis type MS
Chromatography type HILIC
Chromatography system Shimadzu LC-30A
Column Waters XBridge Amide (100 x 4.6mm,3.5um)
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 5600 TripleTOF
Ion Mode NEGATIVE
Units normalized peak area

Chromatography:

Chromatography ID:CH001404
Chromatography Summary:The mobile phase consisted of solvent A (5 mM ammonium acetate, pH=9, and 5% acetonitrile) and solvent B (acetonitrile). The gradient was set as follows: 0–3 min, 85% B; 3–6 min, 85–30% B; 6–15 min, 30–2% B; 15–18 min, 2% B; 18–19 min, 2–85% B; 19–26 min, 85% B. The flow rate was set at 0.4 mL/min.
Instrument Name:Shimadzu LC-30A
Column Name:Waters XBridge Amide (100 x 4.6mm,3.5um)
Flow Gradient:0-3 min, 85% B; 3-6 min, 85-30% B; 6-15 min, 30-2% B; 15-18 min, 2% B; 18-19 min, 2-85% B; 19-26 min, 85% B.
Flow Rate:0.4 mL/min
Solvent A:5% acetonitrile/95% water; 5 mM ammonium acetate, pH 9
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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