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MB Sample ID: SA081567

Local Sample ID:QC_P_2
Subject ID:SU001241
Subject Type:Other
Subject Species:Plasmodium falciparum
Taxonomy ID:5833

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Combined analysis:

Analysis ID AN001950 AN001951
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ZIC-pHILIC (150 x 4.6mm,5um) ZIC-pHILIC (150 x 4.6mm,5um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Signal Intensity Signal Intensity

Chromatography:

Chromatography ID:CH001414
Chromatography Summary:Briefly, samples (10 μL) were injected onto a Dionex RSLC U3000 LC system (Thermo) fitted with a ZIC-pHILIC column (5 μm particle size, 4.6 by 150 mm; Merck) and 20 mM ammonium carbonate (A) and acetonitrile (B) were used as the mobile phases. A 30 min gradient starting from 80% B to 40% B over 20 min, followed by washing at 5% B for 3 min and re-equilibration at 80% B, was used.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ZIC-pHILIC (150 x 4.6mm,5um)
Flow Gradient:A 30 min gradient starting from 80% B to 40% B over 20 min, followed by washing at 5% B for 3 min and re-equilibration at 80% B, was used.
Solvent A:100% water; 20 mM ammonium carbonate
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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