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MB Sample ID: SA086279
| Local Sample ID: | cadmium-4 |
| Subject ID: | SU001289 |
| Subject Type: | Invertebrate |
| Subject Species: | Apis mellifera |
| Taxonomy ID: | 7460 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002035 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity I-Class |
| Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001475 |
| Chromatography Summary: | We analyzed targeted, polar primary metabolites on a Xevo TQ-XS triple quadrupole mass spectrometer (Waters) coupled to an I-class UPLC system (Waters) in the UC Riverside Metabolomics Core Facility. We used a ZIC-pHILIC column (2.1 x 150 mm, 5 µM) (EMD Millipore) for separations with the following mobile phases:(A) water with 15 mM ammonium bicarbonate adjusted to pH 9.6 with ammonium hydroxide and (B) acetonitrile. We set the flow rate to 200 µL/min and held the column 50° C with an injection volume of 1 µL. We used the following gradient: 0 min, 90% B; 1.5 min, 90% B; 16 min, 20% B; 18 min, 20% B; 20 min, 90% B; 28 min, 90% B. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min, 90% B; 1.5 min, 90% B; 16 min, 20% B; 18 min, 20% B; 20 min, 90% B; 28 min, 90% B |
| Flow Rate: | 200 ul/min |
| Solvent A: | 100% water; 15 mM ammonium bicarbonate, pH 9.6 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
