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MB Sample ID: SA086290
Local Sample ID: | selenium-2 |
Subject ID: | SU001289 |
Subject Type: | Invertebrate |
Subject Species: | Apis mellifera |
Taxonomy ID: | 7460 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002035 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity I-Class |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo-TQ-S |
Ion Mode | POSITIVE |
Units | peak area |
Chromatography:
Chromatography ID: | CH001475 |
Chromatography Summary: | We analyzed targeted, polar primary metabolites on a Xevo TQ-XS triple quadrupole mass spectrometer (Waters) coupled to an I-class UPLC system (Waters) in the UC Riverside Metabolomics Core Facility. We used a ZIC-pHILIC column (2.1 x 150 mm, 5 µM) (EMD Millipore) for separations with the following mobile phases:(A) water with 15 mM ammonium bicarbonate adjusted to pH 9.6 with ammonium hydroxide and (B) acetonitrile. We set the flow rate to 200 µL/min and held the column 50° C with an injection volume of 1 µL. We used the following gradient: 0 min, 90% B; 1.5 min, 90% B; 16 min, 20% B; 18 min, 20% B; 20 min, 90% B; 28 min, 90% B. |
Instrument Name: | Waters Acquity I-Class |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 50 |
Flow Gradient: | 0 min, 90% B; 1.5 min, 90% B; 16 min, 20% B; 18 min, 20% B; 20 min, 90% B; 28 min, 90% B |
Flow Rate: | 200 ul/min |
Solvent A: | 100% water; 15 mM ammonium bicarbonate, pH 9.6 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |