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MB Sample ID: SA091103
| Local Sample ID: | 17_23 _t_minus_50 |
| Subject ID: | SU001319 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-27 |
| Weight Or Weight Range: | 58-92 |
| Height Or Height Range: | 172-190 |
| Gender: | Male |
| Human Race: | Caucasian |
| Human Trial Type: | Prospective randomized open label controlled intervention study |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | Non smoker |
| Human Alcohol Drug Use: | Not in |
| Human Nutrition: | Fasted for 12 hours before the first sample was obtained. |
| Human Inclusion Criteria: | Healthy (normal physical examination, electrocardiography, and routine laboratory values). |
| Human Exclusion Criteria: | Febrile illness during the 2 weeks before the endotoxemia experiment, taking any prescription medication, history of spontaneous vagal collapse, practicing or experience with any kind of meditation, or participation in a previous trial where LPS was administered. |
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Combined analysis:
| Analysis ID | AN002077 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Discovery HS F5-3 (15cm x 2.1 mm,3um) Supelco,Sigma Aldrich) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6540 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | relative abundance |
Chromatography:
| Chromatography ID: | CH001514 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Discovery HS F5-3 (15cm x 2.1 mm,3um) Supelco,Sigma Aldrich) |
| Flow Gradient: | 5% B from 0-0.1min to 35% B at 1.5 min, to 95% B at 2.05 min which was kept constant until 3.2 min, to 5% B at 3.21 min and washing until 4.3 min with 5% B. |
| Flow Rate: | The flow rate was kept constant at 700 µL/min from 0 min to 2.2 min, and increased up to 900 µL/min from 2.2 min to 2.5 min, after which flow rate was kept constant until 3.2 min.The flow rate was decreased from 900 µL/min to 800 µL/min from 3.2 min to 3.1 min and kept constant until 3.7 min, when flow rate was changed to 700 µL/min. |
| Injection Temperature: | 40 °C |
| Solvent A: | 95% water/5% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
