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MB Sample ID: SA092007
| Local Sample ID: | MeOH-H2O |
| Subject ID: | SU001333 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | MCF-7 |
| Cell Passage Number: | 30 |
| Species Group: | Homo Sapien |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002102 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GCMS-QP2010 Ultra |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Shimadzu QP2010 Ultra |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001534 |
| Chromatography Summary: | A GC/MS-QP 2010 Ultra System (Shimadzu, Kyoto, Japan) was employed for the metabolomic analysis, along with LabSolutions GC-MS software (ver). A Restek Rtx®-5ms column (30.0 m × 0.25 mm, 0.25 µm) was utilized for separation of the metabolites. Helium (99.9%) was used as the carrier gas at the flow rate of 1.0 mL/min. The initial oven temperature was set at 60 °C and was held at this temperature for 2 minutes, then raised to 310 °C by 50 °C/min and held at this temperature during the analysis. Both the auxiliary temperature at the interface and the ionization temperature were 250 °C. Metabolites were analysed in full scan mode within the range of 50 – 650 amu. Total volume of 10 µL was injected in splitless mode, by employing AOC-20i Auto Injector (Shimadzu, Kyoto, Japan). GC total ion chromatograms (TIC) and fragmentation patterns of the metabolites identified using NIST/EPA/NIH Mass Spectral Library (NIST 14). Run time for each sample was 43.67 min. |
| Methods Filename: | PAH scan |
| Instrument Name: | Shimadzu GCMS-QP2010 Ultra |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Pressure: | 57.4 |
| Column Temperature: | 60 |
| Flow Rate: | 1 |
| Injection Temperature: | 250 |
| Retention Time: | many retention time |
| Sample Injection: | 1 |
| Solvent A: | Pyridine |
| Analytical Time: | 43.67 |
| Oven Temperature: | 60 |
| Time Program: | 43.67 |
| Transferline Temperature: | 250 |
| Strong Wash Solvent Name: | Acetone |
| Strong Wash Volume: | 10 uL |
| Sample Syringe Size: | 10 uL |
| Chromatography Type: | GC |
