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MB Sample ID: SA093130
Local Sample ID: | DMSO_6 |
Subject ID: | SU001347 |
Subject Type: | Cultured cells |
Subject Species: | Trypanosoma brucei brucei |
Taxonomy ID: | 5702 |
Genotype Strain: | 427 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002116 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS |
Column | Ascentis Express C8 (10cm x 2.1mm,2.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Peak height |
Chromatography:
Chromatography ID: | CH001549 |
Chromatography Summary: | Metabolite extracts were analysed using reversed phase liquid chromatography (LC) and high resolution mass spectrometry on an Orbitrap system. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Ascentis Express C8 (10cm x 2.1mm,2.7um) |
Column Temperature: | 40°C |
Flow Gradient: | linear gradient -time, %B as follows: 0min- 0%, 1.5min- 20%, 7min- 28%, 8min- 35%, 24min- 65%, 25min- 100%. |
Flow Rate: | 200 μL/min |
Sample Injection: | 10 μL |
Solvent A: | 40% isopropanol/60% water; 2 mM formic acid; 8 mM ammonium formate |
Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium formate |
Chromatography Type: | Reversed phase |