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MB Sample ID: SA093152
| Local Sample ID: | MMV688179_4 |
| Subject ID: | SU001347 |
| Subject Type: | Cultured cells |
| Subject Species: | Trypanosoma brucei brucei |
| Taxonomy ID: | 5702 |
| Genotype Strain: | 427 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002116 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Ascentis Express C8 (10cm x 2.1mm,2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak height |
Chromatography:
| Chromatography ID: | CH001549 |
| Chromatography Summary: | Metabolite extracts were analysed using reversed phase liquid chromatography (LC) and high resolution mass spectrometry on an Orbitrap system. |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Ascentis Express C8 (10cm x 2.1mm,2.7um) |
| Column Temperature: | 40°C |
| Flow Gradient: | linear gradient -time, %B as follows: 0min- 0%, 1.5min- 20%, 7min- 28%, 8min- 35%, 24min- 65%, 25min- 100%. |
| Flow Rate: | 200 μL/min |
| Sample Injection: | 10 μL |
| Solvent A: | 40% isopropanol/60% water; 2 mM formic acid; 8 mM ammonium formate |
| Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium formate |
| Chromatography Type: | Reversed phase |
