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MB Sample ID: SA093747
| Local Sample ID: | 12HKR4 |
| Subject ID: | SU001363 |
| Subject Type: | Plant |
| Subject Species: | Triticum durum |
| Taxonomy ID: | 4567 |
| Genotype Strain: | cv. Opera |
| Age Or Age Range: | 10 days |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002145 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Trace GC 1310, Thermo Fisher Scientific |
| Column | TG-5MS |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | ISQ LT, Thermo Fisher Scientific |
| Ion Mode | POSITIVE |
| Units | Relative Abundance |
Chromatography:
| Chromatography ID: | CH001570 |
| Chromatography Summary: | The derivatized extracts were injected into a TG-5MS capillary column (30 m x 0.25 mm x 0.25 µm) (Thermo Fisher Scientific, Waltham, MA, USA) using a gas chromatograph apparatus (Trace GC 1310, Thermo Fisher Scientific, Waltham, MA, USA) equipped with a single quadrupole mass spectrometer (ISQ LT, Thermo Fisher Scientific, Waltham, Massachusetts, US). Injector and source were set at 250°C and 260°C temperature, respectively. One µl of sample was injected in splitless mode with a helium flow of 1 ml/min using the following programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C. |
| Methods ID: | NA |
| Instrument Name: | Trace GC 1310, Thermo Fisher Scientific |
| Column Name: | TG-5MS |
| Column Temperature: | programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 250 |
| Internal Standard: | Adonitol (0.0002mg/ml of pure water) 60 μL per sample |
| Retention Index: | Alkanes mixture C10-C40 |
| Retention Time: | Alkanes mixture C10-C40 |
| Oven Temperature: | programmed temperature: isothermal 5 min at 70 °C followed by a 5°C/ min ramp to 350 °C and a final 5 min heating at 330°C |
| Transferline Temperature: | 250 |
| Sample Syringe Size: | 10 μL |
| Randomization Order: | YES |
| Chromatography Type: | GC |
