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MB Sample ID: SA096444
| Local Sample ID: | JW47-LF |
| Subject ID: | SU001403 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 5-6 months or 26 months |
| Animal Animal Supplier: | NIA |
| Animal Housing: | UW ARCF |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002215 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu Nexera XR LC-20AD |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001625 |
| Chromatography Summary: | Samples were injected into a chromatography system consisting of a dual injection valve setup allowing injections onto two different LC columns with each column dedicated to an ESI polarity. 5 µL were injected on the positive mode column and 10 µL on the negative side column. The columns were a matched pair from the same production lot number and were both a Waters XBridge BEH amide column (2.1 x 150 mm). Autosampler was maintained at 4 °C and column oven was set to 40 °C. 0.3 mL/min gradient was 0-3 min 95% B, 3-8 min 95 -> 50% B, 8-12 min 50% B, 12-13 min 50 – 95% B, 13-18 min 95% B. After completion of the 18 minute gradient, injection on the opposite column was initiated and the inactive column was allowed to equilibrate at starting gradient conditions. A set of QC injections for both instrument and sample QC were run at the beginning and end of the sample run. |
| Instrument Name: | Shimadzu Nexera XR LC-20AD |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Solvent A: | 95% water/3% acetonitrile/2% methanol; 0.2% acetic acid; 10 mM ammonium acetate |
| Solvent B: | 93% acetonitrile/5% water/2% methanol; 0.2% acetic acid; 10 mM ammonium acetate |
| Chromatography Type: | HILIC |
