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MB Sample ID: SA096485
| Local Sample ID: | OXGF14b-4-24h-2 |
| Subject ID: | SU001404 |
| Subject Type: | Plant |
| Subject Species: | Oryza sativa Japonica Group |
| Taxonomy ID: | 39947 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002216 | AN002217 | AN002218 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Agilent DB35-MS (30m x 0.25mm,0.25um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Orbitrap | Orbitrap |
| MS instrument name | Agilent 5975 | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE |
| Units | Relative content | Relative content | Relative content |
Chromatography:
| Chromatography ID: | CH001626 |
| Chromatography Summary: | One µl was taken from each sample and injected into GC-MS at 270°C in a split mode (50: 1) with helium carrier gas (> 99.999% purity) flow set to 1 ml/min, and separated by a DB-35MS UI (30 m × 0.25 mm, 0.25 µm) capillary column. The temperature was isothermal for 4 min at 90°C, followed by an 8°C per min ramp up to 205°C, then held for 2 min, and finally ramped up at a rate of 15°C per min to 310°C, held for 2 min. |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB35-MS (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
| Chromatography ID: | CH001627 |
| Chromatography Summary: | Firstly, 10 μL of each sample was eluted using a TSS T3 column (100 mm × 2.1 mm containing 1.8 μm diameter particles, Waters) with 0.4 mL/min flow rate. The mobile phase A was water with 0.1% formic acid, and the mobile phase B was ACN with 0.1% formic acid. The compounds were separated by a elution gradient: 1% B was initially firstly maintained for 1 min, then linearly increased to 40% B from 1 to 11 min, to 70% B from 11 to 13 min, then to 99% B from 13 to 15 min, and maintained at 99% B from 15 to 16 min, then linearly decreased to 1% B from 16 to 17 min followed by equilibration at 1% B for 3 min. and the column temperature was set at 40°C. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40°C |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
