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MB Sample ID: SA096516
| Local Sample ID: | OXGF14b-2-24h-3 |
| Subject ID: | SU001405 |
| Subject Type: | Plant |
| Subject Species: | Oryza sativa Japonica Group |
| Taxonomy ID: | 39947 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002219 | AN002220 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters CORTECS C18 (150 x 2.1mm,2.7um) | Waters CORTECS C18 (150 x 2.1mm,2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Relative content | Relative content |
Chromatography:
| Chromatography ID: | CH001628 |
| Chromatography Summary: | Five μL of each sample was eluted using a CORTECS® C18 column (150 mm × 2.1 mm, 2.7μm, Waters) with 0.4 mL/min flow rate. The mobile phase A was water: ACN (40: 60, v/v) with 0.1% formic acid and 10mM ammonium formate, and the mobile phase B was 2-propanol: ACN (90:10, v/v) with 0.1% formic acid and 10mM ammonium formate. The compounds were separated by a elution gradient: 20% B was firstly maintained for 0.2 min, then linearly decreased to 60% B from 0.2 to 2min, to 100% B from 2 to 9min, and maintained at 100% B from 9 to 10 min, then linearly increased to 20% B from 10 to 10.5 min followed by equilibration at 20% B for 3.5 min. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters CORTECS C18 (150 x 2.1mm,2.7um) |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
