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MB Sample ID: SA098184
| Local Sample ID: | Con013 |
| Subject ID: | SU001427 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6J |
| Age Or Age Range: | 18-20 weeks |
| Weight Or Weight Range: | 20-30g |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | 5/cage |
| Animal Light Cycle: | 12h |
| Animal Feed: | Ad libitum. Control mice received custom casein-diet. Chronic kidney disease was induced by supplementing casein-based diet with 0.15% adenine |
| Animal Water: | Ad libitum |
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Combined analysis:
| Analysis ID | AN002251 | AN002252 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex | Thermo Dionex |
| Column | ACE 5 C18-300 (100 x 2.1mm) | ACE 5 C18-300 (100 x 2.1mm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH001655 |
| Chromatography Summary: | samples were processed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative heated electrospray ionization with a mass resolution of 35,000 at m/z 200 as separate injections. Separation was achieved on an ACE 18-pfp 100 x 2.1 mm, 2 µm column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. This is a polar embedded stationary phase that provides comprehensive coverage, but does have some limitation is the coverage of very polar species. The flow rate was 350 µL/min with a column temperature of 25°C. 4 µL was injected for negative ions and 2 µL for positive ions. |
| Instrument Name: | Thermo Dionex |
| Column Name: | ACE 5 C18-300 (100 x 2.1mm) |
| Column Temperature: | 25C |
| Flow Rate: | 350ul/min |
| Sample Injection: | 4ul for negative ion, 2ul for positive ion |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
