Return to study ST001353 main page
MB Sample ID: SA098186
Local Sample ID: | Con018 |
Subject ID: | SU001427 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6J |
Age Or Age Range: | 18-20 weeks |
Weight Or Weight Range: | 20-30g |
Gender: | Male and female |
Animal Animal Supplier: | Jackson Laboratories |
Animal Housing: | 5/cage |
Animal Light Cycle: | 12h |
Animal Feed: | Ad libitum. Control mice received custom casein-diet. Chronic kidney disease was induced by supplementing casein-based diet with 0.15% adenine |
Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002251 | AN002252 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex | Thermo Dionex |
Column | ACE 5 C18-300 (100 x 2.1mm) | ACE 5 C18-300 (100 x 2.1mm) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH001655 |
Chromatography Summary: | samples were processed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. All samples were analyzed in positive and negative heated electrospray ionization with a mass resolution of 35,000 at m/z 200 as separate injections. Separation was achieved on an ACE 18-pfp 100 x 2.1 mm, 2 µm column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. This is a polar embedded stationary phase that provides comprehensive coverage, but does have some limitation is the coverage of very polar species. The flow rate was 350 µL/min with a column temperature of 25°C. 4 µL was injected for negative ions and 2 µL for positive ions. |
Instrument Name: | Thermo Dionex |
Column Name: | ACE 5 C18-300 (100 x 2.1mm) |
Column Temperature: | 25C |
Flow Rate: | 350ul/min |
Sample Injection: | 4ul for negative ion, 2ul for positive ion |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |