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MB Sample ID: SA098554
Local Sample ID: | S2R1 |
Subject ID: | SU001430 |
Subject Type: | Other |
Subject Species: | Ilyonectria mors-panacis;Ilyonectria rufa;Ilyonectria robusta;Ilyonectria torresensis;Ilyonectria estremocensis;Neonectria obtusispora |
Taxonomy ID: | 1079131;1079113;1079257;1199091;1079267;78417 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002255 | AN002256 | AN002257 | AN002258 | AN002259 |
---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC | HILIC |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
Column | Waters Acquity UPLC HSS Cyano (CN) ( 50 x 2.1mm,1.8um) | Waters Acquity UPLC HSS Cyano (CN) ( 50 x 2.1mm,1.8um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Orbitrap | Triple quadrupole | Triple quadrupole | Orbitrap |
MS instrument name | Waters Xevo-TQ-S | Thermo Q Exactive HF hybrid Orbitrap | Waters Xevo-TQ-S | Waters Xevo-TQ-S | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE | POSITIVE |
Units | Normalized Peak Area Per L Seawater Filtered | Normalized Peak Area Per L Seawater Filtered | Normalized Peak Area Per L Seawater Filtered | Normalized Peak Area Per L Seawater Filtered | Normalized Peak Area per L of SW filtered |
Chromatography:
Chromatography ID: | CH001657 |
Chromatography Summary: | RP |
Methods Filename: | Ingalls_Metabolomics_MS_Parameters_2015.pdf |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity UPLC HSS Cyano (CN) ( 50 x 2.1mm,1.8um) |
Column Temperature: | 35 C |
Flow Gradient: | 5% B for 2 minutes, ramped to 100% B over 16 minutes, held at 100% B for 2 minutes, and equilibrated at 5% B for 5 minutes |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 20 min |
Preconditioning: | equilibrated at the starting conditions for at least 10 minutes; several water blanks were run before |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001658 |
Chromatography Summary: | HILIC |
Methods Filename: | Ingalls_Metabolomics_MS_Parameters_2015.pdf |
Chromatography Comments: | In marine samples, a major salt peak elutes at approximately 23 minutes, To improve the performance of the HILIC column, we maintained the same injection volume, kept the instrument running water blanks between samples as necessary. |
Instrument Name: | Waters Acquity I-Class |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 30 C |
Flow Gradient: | 100% A for 2 minutes, ramped to 100% B over 18 minutes, held at 100% B for 5 minutes, and equilibrated at 100% A for 25 minutes |
Flow Rate: | 0.15 mL/min |
Solvent A: | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
Solvent B: | 40% acetonitrile/60% water; 10 mM ammonium carbonate |
Analytical Time: | 25 min |
Preconditioning: | , the column was equilibrated at the starting conditions for at least 30 minutes |
Chromatography Type: | HILIC |