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MB Sample ID: SA100082
Local Sample ID: | 20191201-A+O Commercial samples-A1-3 |
Subject ID: | SU001448 |
Subject Type: | Other |
Subject Species: | Malus domestica;Citrus sinensis |
Taxonomy ID: | 3750;2711 |
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Combined analysis:
Analysis ID | AN002294 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | SCIEX ExionLC |
Column | Agilent Eclipse Plus C18,RRHD (100 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple TOF |
MS instrument name | ABI Sciex 6600 TripleTOF |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH001685 |
Chromatography Summary: | All samples were subjected to reversed-phase chromatography using a UHPLC system, including ExionLC solvent valve, ExionLC AD pump, ExionLC autosampler, ExionLC controller, ExionLC AC column oven (SCIEX, Redwood City, CA, USA). Mobile phases A was 0.2% formic acid in water and B was acetonitrile, respectively. Chromatographic separation was performed at a flow rate of 300 μL/min using a UHPLC column (Eclipse Plus C18, RRHD 1.8 μm, 2.1*100 mm, Agilent, USA) maintained at 40 °C. Solvent gradient was as follow: 0−10 min, 5%−35% B; 10−12 min, 35−100% B; 12−15 min, 100% B; 15−15.01 min, 100%−5% B; 15.01−18 min, 5% B. Injection volume was 2 μL for all samples. |
Instrument Name: | SCIEX ExionLC |
Column Name: | Agilent Eclipse Plus C18,RRHD (100 x 2.1mm,1.8um) |
Flow Gradient: | 0-10 min, 5%-35% B; 10-12 min, 35-100% B; 12-15 min, 100% B; 15-15.01 min, 100%-5% B; 15.01-18 min, |
Flow Rate: | 300ul/min |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |