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MB Sample ID: SA113896
Local Sample ID: | WT at 3hr minR+Leu Sample1 |
Subject ID: | SU001475 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4743 |
Gender: | Not applicable |
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Combined analysis:
Analysis ID | AN002343 |
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Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 5977b |
Column | Phenomenex Zebron AB-5HT (5m) |
MS Type | EI |
MS instrument type | HES-MSD |
MS instrument name | Agilent 5977 |
Ion Mode | POSITIVE |
Units | AUC (unitless) |
Chromatography:
Chromatography ID: | CH001716 |
Chromatography Summary: | All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 10:1 split ratio was used for analysis of the majority of metabolites. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 5 minutes. A 30-meter Phenomenex Zebron AB-5HT with 5m inert Guardian capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. |
Instrument Name: | Agilent 5977b |
Column Name: | Phenomenex Zebron AB-5HT (5m) |
Chromatography Type: | GC |