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MB Sample ID: SA113898
| Local Sample ID: | WT at 3hr minR+Leu Sample3 |
| Subject ID: | SU001475 |
| Subject Type: | Yeast |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
| Genotype Strain: | BY4743 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002343 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 5977b |
| Column | Phenomenex Zebron AB-5HT (5m) |
| MS Type | EI |
| MS instrument type | HES-MSD |
| MS instrument name | Agilent 5977 |
| Ion Mode | POSITIVE |
| Units | AUC (unitless) |
Chromatography:
| Chromatography ID: | CH001716 |
| Chromatography Summary: | All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 10:1 split ratio was used for analysis of the majority of metabolites. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 5 minutes. A 30-meter Phenomenex Zebron AB-5HT with 5m inert Guardian capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. |
| Instrument Name: | Agilent 5977b |
| Column Name: | Phenomenex Zebron AB-5HT (5m) |
| Chromatography Type: | GC |
