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MB Sample ID: SA114105
| Local Sample ID: | LIPNEG17MAR_42.BLANK3 |
| Subject ID: | SU001479 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002347 | AN002348 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Prominence 20 UFLCXR | Shimadzu Prominence 20 UFLCXR |
| Column | Waters XBridge C18 (50 x 2.1mm,3um) | Waters XBridge C18 (50 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | ppm | ppm |
Chromatography:
| Chromatography ID: | CH001720 |
| Chromatography Summary: | Samples were separated by reverse phase HPLC using a Prominence 20 UFLCXR system (Shimadzu, Columbia MD) with a Waters (Milford, MA) CSH C18 column (100mm x 2.1mm 1.7 um particle size) maintained at 55C and a 20 minute aqueous/acetonitrile/isopropanol gradient, at a flow rate of 225 ul/min. For electrospray ionization positive mode, Solvent A was 40% water, 60% acetonitrile with 10mM ammonium formate and 0.1% formic acid, and Solvent B was 90% isopropanol, 10% acetonitrile with 10mM ammonium formate and 0.1% formic acid. For electrospray ionization negative mode, Solvent A was 40% water, 60% isopropanol with 10 mM ammonium acetate, and solvent B was 90% isopropanol, 10% acetonitrile with 10 mM ammonium acetate. The initial conditions were 60% A and 40% B, increasing to 43% B at 2 minutes, 50% B at 2.1 minutes, 54% B at 12 minutes, 70% B at 12.1 minutes and 99% B at 18 minutes, held at 99% B until 20.0 minutes before returning to the initial conditions. |
| Instrument Name: | Shimadzu Prominence 20 UFLCXR |
| Column Name: | Waters XBridge C18 (50 x 2.1mm,3um) |
| Column Temperature: | 55 |
| Flow Gradient: | The initial conditions were 60% A and 40% B, increasing to 43% B at 2 minutes, 50% B at 2.1 minutes, 54% B at 12 minutes, 70% B at 12.1 minutes and 99% B at 18 minutes, held at 99% B until 20.0 minutes before returning to the initial conditions. |
| Flow Rate: | 225 ul/min |
| Solvent A: | Pos mode: 40% water/60% acetonitrile; 0.1% formic acid; 10mM ammonium formate, Neg mode: 40% water/60% isopropanol; 10 mM ammonium acetate |
| Solvent B: | Pos mode: 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate, Neg mode: 90% isopropanol/10% acetonitrile; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
