Return to study ST001420 main page

MB Sample ID: SA117945

Local Sample ID:S102
Subject ID:SU001494
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:59 ± 14.6
Gender:Male and female
Human Ethnicity:Asian
Human Medications:All with aspirin and clopidogrel
Human Smoking Status:22.2% were smokers
Human Inclusion Criteria:The inclusion criteria were hospitalized CAD patients with stable angina.
Human Exclusion Criteria:Patient with the lesion of > 90% stenosis or total occlusion, active inflammation (High sensitive C-reactive protein [hsCRP] >1.0 mg/dl), renal disease (serum creatinine > 3.0 mg/dl or ongoing hemodialysis), the onset of cancer in the previous five years, coronary artery bypass grafting, contraindications to iodine media, and severe hepatic insufficiency.

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002375
Analysis type MS
Chromatography type HILIC
Chromatography system Shimadzu 20AD
Column Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm)
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 6500 QTrap
Units Peak area


Chromatography ID:CH001743
Chromatography Summary:Metabolomic analysis was performed on a Shimadzu LC-20A ultra-high-performance liquid chromatography coupled with SCIEX QTRAP 6500+ triple quadrupole mass spectrometer (LC-MS/MS) using a broad-spectrum targeted metabolomic approach. Ten µL of the extract was injected into a 250 mm × 2.0 mm, 4 µm polymer-based NH2 HPLC column (Asahipak NH2P-40 2E, Showa Denko America, Inc., NY) held at 25°C for chromatographic separation. The mobile phase was solvent A: 95% water, 5% acetonitrile with 20 mM NH4OAC, and 20 mM NH4OH (pH 9.4); solvent B: 100% acetonitrile. Separation was achieved using the following gradient: 0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end. The flow rate was 250 µL/min.
Instrument Name:Shimadzu 20AD
Column Name:Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm)
Column Temperature:25 ℃
Flow Gradient:0-3.5 min: 95% B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 14-30 min: 0% B, 31-41 min: 95% B, 41.1 min: end.
Flow Rate:250 µL/min
Solvent A:95% water/5% acetonitrile; 20 mM ammonium hydroxide, pH 9.4 (adjusted by formic acid)
Solvent B:100% acetonitrile
Chromatography Type:HILIC