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MB Sample ID: SA124451
| Local Sample ID: | TB_5m_2 |
| Subject ID: | SU001541 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002443 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Zorbax Eclipse XDB-C18 (100 x 2mm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | area ratio |
Chromatography:
| Chromatography ID: | CH001788 |
| Chromatography Summary: | Metabolites related to glucose-mediated metabolites were analyzed with LC-MS/MS (MRM) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Synergi fusion RP (50 x 2mm) |
| Column Temperature: | 23 |
| Flow Gradient: | 0% B for 5 min, 0% to 90% B for 2 min, hold at 90% B for 8 min, 90% to 0% B for 1 min, and then hold at 0% B for 9 min |
| Flow Rate: | 70 μL/min except for minutes 7 to 15, when it was 140 µL/min |
| Internal Standard: | 13C5-glutamine |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 100% acetonitrile; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001789 |
| Chromatography Summary: | Amino acids and bioamines were analyzed with LC-MS/MS (MRM) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Zorbax Eclipse XDB-C18 (100 x 2mm) |
| Column Temperature: | 50 |
| Flow Gradient: | 0% B for 0.5 min, 0% to 95% B for 5 min, hold at 95% B for 1 min, 95% to 0% B for 0.5 min, then hold at 0% B for 2.5 min |
| Flow Rate: | 500 μL/min |
| Internal Standard: | 13C5-glutamine, serotonine-d4, dopamine-d4, tryptophan-d5, serine-d3, and lysine-d8 |
| Sample Injection: | 3 uL |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
