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MB Sample ID: SA125678
| Local Sample ID: | 13206198 |
| Subject ID: | SU001564 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 18-45 |
| Gender: | Male and female |
| Human Nutrition: | Low glycemic load and high glycemic load diet |
| Human Inclusion Criteria: | We recruited non-smoking, healthy individuals between the ages of 18-45 years from the Greater Seattle area. |
| Human Exclusion Criteria: | Exclusion criteria consisted of impaired fasting glucose (fasting blood glucose ≥5.6 mmol/L), any physician-diagnosed condition requiring a restricted diet, food allergies, regular use of hormones or anti-inflammatory medication, current pregnancy or lactation or plans to become pregnant, or heavy use of alcohol (>2 drinks/d) |
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Combined analysis:
| Analysis ID | AN002468 | AN002469 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | Triple quadrupole | Triple quadrupole |
| Column | ESI | ESI |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | mM | mM |
Chromatography:
| Chromatography ID: | CH001809 |
| Chromatography Summary: | Quantitative lipidomics was performed with the Sciex Lipidyzer platform consisting of Shimadzu Nexera X2 LC-30AD pumps, a Shimadzu Nexera X2 SIL-30AC autosampler, and a Sciex QTRAP® 5500 mass spectrometer equipped with SelexION® for differential mobility spectrometry (DMS). 1-propanol was used as the chemical modifier for the DMS. Samples were introduced to the mass spectrometer by flow injection analysis at 8 uL/min aka direct infusion. Each sample was injected twice, once with the DMS on Method 1 (PC/PE/LPC/LPE/SM), and once with the DMS off Method 2 (CE/CER/DAG/DCER/FFA/HCER/LCER/TAG). The lipid molecular species were measured using multiple reaction monitoring (MRM) and positive/negative polarity switching. Positive ion mode detected lipid classes SM/DAG/CE/CER/DCER/HCER/LCER/TAG and negative ion mode detected lipid classes LPE/LPC/PC/PE/FFA. A total of 1070 lipids and fatty acids were targeted in the analysis. |
| Instrument Name: | Triple quadrupole |
| Column Name: | ESI |
| Chromatography Type: | None (Direct infusion) |
