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MB Sample ID: SA136026

Local Sample ID:2 - Feces_fldC-3_031
Subject ID:SU001683
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN002639
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890 GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25m)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus IV TOF
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH001949
Chromatography Summary:Injector conditions: Agilent 6890 GC is equipped with a Gerstel automatic liner exchange system (ALEX) that includes a multipurpose sample (MPS2) dual rail, and a Gerstel CIS cold injection system (Gerstel, Muehlheim, Germany) with temperature program as follows: 50°C to 275°C final temperature at a rate of 12 °C/s and hold for 3 minutes. Injection volume is 0.5 μl with 10 μl/s injection speed on a splitless injector with purge time of 25 seconds. Liner (Gerstel #011711-010-00) is changed after every 10 samples, (using the Maestro1 Gerstel software vs. 1.1.4.18). Before and after each injection, the 10 μl injection syringe is washed three times with 10 μl ethyl acetate. Gas Chromatography conditions: A 30 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film) with additional 10 m integrated guard column is used (Restek, Bellefonte PA). 99.9999% pure Helium with built-in purifier (Airgas, Radnor PA) is set at constant flow of 1 ml/min. The oven temperature is held constant at 50°C for 1 min and then ramped at 20°C/min to 330°C at which it is held constant for 5 min.
Chromatography Comments:0 m long, 0.25 mm i.d. Rtx-5Sil MS column (0.25 μm 95% dimethyl 5% diphenyl polysiloxane film))
Instrument Name:Agilent 6890 GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25m)
Chromatography Type:GC
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