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MB Sample ID: SA151946

Local Sample ID:F5
Subject ID:SU001735
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932
Cell Strain Details:BY4741

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002706 AN002707 AN002708 AN002709
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Normal phase Normal phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Thermo Hypersil Gold aQ (150 x 2.1mm,1.9um) Thermo Hypersil Gold aQ (150 x 2.1mm,1.9um) PolyLC Polyhydroxyethyl A (200 x 2.1mm,5um) PolyLC Polyhydroxyethyl A (200 x 2.1mm,5um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak Area Peak Area

Chromatography:

Chromatography ID:CH001997
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Thermo Hypersil Gold aQ (150 x 2.1mm,1.9um)
Column Temperature:30
Flow Gradient:0-2min 0% B, 2-13min 0-60% B, 13-15min 60-90% B, 15-16min 90-0% B, 16-20min 0% B
Flow Rate:0.2 mL/min
Solvent A:100% water; 0.1% formic acid; 1mM ammonium acetate
Solvent B:20% water/80% acetonitrile; 0.1% formic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH001998
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:PolyLC Polyhydroxyethyl A (200 x 2.1mm,5um)
Column Temperature:30
Flow Gradient:0-2min 80% B, 2-13min 80-40% B, 13-15min 40-10% B, 15-16min 10-80% B, 16-20min 80% B
Flow Rate:0.2 mL/min
Solvent A:100% water; 0.1% formic acid; 1mM ammonium acetate
Solvent B:20% water/80% acetonitrile; 0.1% formic acid; 1 mM ammonium acetate
Chromatography Type:Normal phase
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