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MB Sample ID: SA155122
| Local Sample ID: | s87_1 |
| Subject ID: | SU001759 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-87 |
| Gender: | Male and female |
| Human Race: | Caucasian |
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Combined analysis:
| Analysis ID | AN002746 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Other |
| MS instrument name | Shimadzu LCMS-IT-TOF |
| Ion Mode | POSITIVE |
| Units | area |
Chromatography:
| Chromatography ID: | CH002028 |
| Chromatography Summary: | The apparatus consisted of a two LC-20AD pumps (Shimadzu, Japan), an automatic sample injector, coupled on-line with an LCMS-IT-TOF (Shimadzu, Japan) mass spectrometer with an electrospray ionization source. The HPLC separation was conducted on a 2.1 × 100 mm Waters Acquity UPLC BEH; 1.7 μm column (Waters, Milford MA, USA) with 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column (Agilent Technologies, USA). Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5% solvent B, elution was performed according to the following conditions: from 5% B to 40% B in 20 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. Total analysis time was 50 min. Column temperature was 35˚C. Autosampler tray temperature was 4˚C. |
| Instrument Name: | Shimadzu 20AD |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Pressure: | 300-500 bar |
| Column Temperature: | 35 |
| Flow Gradient: | non-linear |
| Flow Rate: | 0.3 ml/min |
| Injection Temperature: | 5 |
| Internal Standard: | papaverine |
| Sample Injection: | 5 mkl |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 50 min |
| Chromatography Type: | Reversed phase |
