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MB Sample ID: SA156794
| Local Sample ID: | C-23MQ1-BR-00 |
| Subject ID: | SU001769 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002762 | AN002763 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Higgins Analytical Targa C18 (50 x 2.1mm,5um) | Higgins Analytical Targa C18 (50 x 2.1mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q-Exactive HFX | Thermo Q-Exactive HFX |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak intensity | Peak intensity |
Chromatography:
| Chromatography ID: | CH002042 |
| Chromatography Summary: | Sample extracts were analyzed using an ultra-high performance liquid chromatography (UHPLC) equipped with dual pumps for both C18-pos and C18-negative analysis uysing a Thermo Scientific Vanquish Duo LC interfaced to a Thermo Scientific Q-Exactive HFX with electrospray ionization source operated in positive mode. Samples were maintained at 4C in the autosampler module. For C18-pos separation, 5 uL of sample was injected onto a C18 columns (50 mm × 2.1 mm, 5 µm particle size, Higgins Analytical Inc) maintained at 30C. Separation occurred using Mobile phase A consisted of water with 0.1% formic acid and Mobile phase B consisted of Acetonitrile with 0.1% formic acid. Flow rate was held at at 0.4 ml/min for 1.5 mins, and increased to 0.5 ml/min. Solvent gradients were as follows: 85% solvent A, hold for 1.5 min; linear decrease to 5% solvent A at 5 minutes; hold for 2.5 min, for a total run time of 7.5 min Data was acquired with a mass range of 85-1275 m/z. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Higgins Analytical Targa C18 (50 x 2.1mm,5um) |
| Column Temperature: | 30C |
| Flow Rate: | 0.4-0.5 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 7.5 min |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002043 |
| Chromatography Summary: | Sample extracts were analyzed using an ultra-high performance liquid chromatography (UHPLC) equipped with dual pumps for both C18-pos and C18-negative analysis using a Thermo Scientific Vanquish Duo LC interfaced to a Thermo Scientific Q-Exactive HFX with electrospray ionization source operated in negative mode. Samples were maintained at 4C in the autosampler module. For C18-neg separation, 5 uL of sample was injected onto a C18 columns (50 mm × 2.1 mm, 5 µm particle size, Higgins Analytical Inc) maintained at 30C. Separation occurred using Mobile phase A consisted of water with 10mM ammonium acetate and Mobile phase B consisted of Acetonitrile. Flow rate was held at at 0.4 ml/min for 1.5 mins, and increased to 0.5 ml/min. Solvent gradients were as follows: 85% solvent A, hold for 1.5 min; linear decrease to 5% solvent A at 5 minutes; hold for 2.5 min, for a total run time of 7.5 min Data was acquired with a mass range of 85-1275 m/z. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Higgins Analytical Targa C18 (50 x 2.1mm,5um) |
| Column Temperature: | 30C |
| Flow Rate: | 0.4-0.5 mL/min |
| Solvent A: | 100% water; 10mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 7.5 min |
| Chromatography Type: | Reversed phase |
