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MB Sample ID: SA164341

Local Sample ID:blank2
Subject ID:SU001843
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN002873
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Phenomenex Luna NH2 (150 x 2mm,3um)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Units ppm


Chromatography ID:CH002128
Chromatography Summary:LUNA-NH2 chromatography: One  ml of reconstituted sample was injected into a Luna® 3 µm NH2 100 Å, LC Column (150x2 mm, 3 µm particle size; Phenomenex, 00F-4377-B0) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 5 mM ammonium acetate and 0.2% ammonium hydroxide. Gradient conditions were: 20 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25–26 min from 90% to 10% B; 26–34 min hold at 10% B. The column oven and autosampler tray were held at 30 °C and 4 °C, respectively
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Luna NH2 (150 x 2mm,3um)
Column Temperature:30
Flow Gradient:0 min linear gradient from 10% to 90% B; 20-25 min hold at 90% B; 25-26 min from 90% to 10% B; 26-34 min hold at 10% B.
Solvent A:100% acetonitrile
Solvent B:100% water; 5 mM ammonium acetate; 0.2% ammonium hydroxide
Chromatography Type:HILIC