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MB Sample ID: SA164366
| Local Sample ID: | kidney3 |
| Subject ID: | SU001844 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002874 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Accucore 150 Amide HILIC (150x3mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | ppm |
Chromatography:
| Chromatography ID: | CH002129 |
| Chromatography Summary: | Accucore-HILIC chromatography: One ml of reconstituted sample was injected into a Thermo Fisher Scientific™ Accucore™ 150 Amide HILIC (150x3 mm, 2.6 mm particle size; Thermo Fisher Scientific) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 20 mM ammonium carbonate, 0.1% ammonium hydroxide. Gradient conditions were: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. The column oven and autosampler tray were held at 35 °C and 4 °C, respectively. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Accucore 150 Amide HILIC (150x3mm,2.6um) |
| Column Temperature: | 35 |
| Flow Gradient: | linear gradient from 20% to 80% B; 20-20.5 min: from 80% to 20% B; 20.5-28 min: hold at 20% B. |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide |
| Chromatography Type: | HILIC |
